Detection of Mycobacterium avium subspecies paratuberculosis excretion in bovine feces, using quantitative real time polymerase chain reaction (Q-PCR)

Detection of Mycobacterium avium subspecies paratuberculosis using nested polymerase chain reaction (nPCR)

Mycobacterium avium subspecies paratuberculosis, the cause of paratuberculosis (Johne’s disease) is a chronic debilitating infection in ruminants. The disease is one of the most widespread bacterial infections of ruminants throughout the world. Recently, the organism was reported to be associated with enteric infection in humans and hence the disease is of public health importance. In the prese...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Detection of Mycobacterium Paratuberculosis using polymerase chain reaction (PCR) in cow raw milk samples in shahre-kord

Abstract Background and objectives: Paratuberculosis or John's disease is a chronic infectious disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). It results in major economic losses to dairy farm of all over the world and it is the agent causing crohn's disease. The aim of this study was to detect the MAP using PCR in raw-milk samples of cows in shahre-kord. Mater...

Comparison of fecal culture and F57 real-time polymerase chain reaction for the detection of Mycobacterium avium subspecies paratuberculosis in Swiss cattle herds with a history of paratuberculosis

BACKGROUND Bovine paratuberculosis is an incurable chronic granulomatous enteritis caused by Mycobacterium avium subspecies paratuberculosis (MAP). The prevalence of MAP in the Swiss cattle population is hard to estimate, since only a few cases of clinical paratuberculosis are reported to the Swiss Federal Food Safety and Veterinary Office each year.Fecal samples from 1,339 cattle (855 animals ...

Direct detection of Mycobacterium avium subsp. Paratuberculosis in bovine milk by multiplex Real-time PCR

The study aimed at direct detection of Mycobacterium avium subsp. Paratuberculosis (MAP) in milk by evaluating a multiplex real-time PCR assay targeting IS900 and ISMAV2 sequences including the amplification of PUC19-plasmid as internal control. The sensitivity of the assays was evaluated by testing MAP isolates in broad linear range of DNA (50 ng – 5 fg/μl). For the validation of the specifici...